Characterization of a Human In Vitro Intestinal Model for the Hazard Assessment of Nanomaterials Used in Cancer Immunotherapy

There is momentum in biomedical research to improve the structure and function of vitro intestinal models that better represent human biology. To build a more comprehensive model, three cell-types were co-cultured characterized: i.e., HT29-MTX (intestinal mucous-producing goblet cells), Caco-2 (colon epithelial Raji B (lymphocytes). cells transformed subpopulation into phagocytic transcytotic immune-supporting microfold (M-cells). A suite bioassays was implemented investigate steady-state barrier integrity cellular communication. The model demonstrated potentiating effect metabolism pro-inflammatory markers. Barrier cell seeding density seem play role reliability endpoint readouts. Microscopic analysis elucidated importance multi-cell biomimicry. data show monocultures do not have same characteristics inherent triple culture models. Multiple types an produce representation intact organ aid ability assess immunomodulatory effects nanomaterials designed for cancer theranostics after ingestion. As many national international agencies stressed, there critical need alternative-to-animal strategies pharmaceuticals effort reduce animal testing.